N-ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism

Table 1 Identification of GST-N-SERT and GST-C-SERT pulled-down proteins from mouse brain extracts

Spot number	Gene name	Protein name	MW (Da)	Number	Sequence coverage	Score	Accession number	N-terminal specific	Cellular and molecular events
N-1	Synj1	Synaptojanin 1	172,509	23	14%	785	Q8CHC4	※	Endocytosis
N-2	Cand1	Cullin-associated NEDD8-dissociated protein 1	136,245	14	10%	526	Q6ZQ38	※	SCF complex assembly
N-3	Aco2	Aconitate hydratase, mitochondrial	85,410	14	19%	534	Q99KI0
N-4	Nsf	Vesicle-fusing ATPase (NSF)	82,561	24	27%	1,010	P46460	※
N-5	Atp6v1a	V-type proton ATPase catalytic subunit A	68,283	13	21%	466	P50516	※	Hydrolysis
N-6	Crmp1	Dihydropyrimidinase-related protein 1	62,129	12	20%	441	P97427	※	Axon guidance and cell migration
N-7	Cct2	T-complex protein 1 subunit beta	57,441	11	19%	202	P80314	※	Molecular chaperone
N-8	Fscn1	Fascin	54,474	14	25%	174	Q61553	※	Actin filament binding
N-9	Eno1	Alpha-enolase	47,111	16	24%	703	P17182
N-10	Cnp	2′,3′-cyclic-nucleotide 3′-phosphodiesterase	47,094	22	40%	341	P16330
C-1	Aco2	Aconitate hydratase, mitochondrial	85,410	8	9%	287	Q99KI0
C-2	Eno1	Alpha-enolase	47,111	8	20%	384	P17182
C-3	Cnp	2′,3′-cyclic-nucleotide 3′-phosphodiesterase	47,094	18	32%	225	P16330

C-SERT, C-terminal domain of the serotonin transporter; GST, glutathione S-transferase; MW, molecular weight; N-SERT, N-terminal domain of the serotonin transporter.

ISSN: 2040-2392