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Fig. 5 | Molecular Autism

Fig. 5

From: Impaired neurogenesis and neural progenitor fate choice in a human stem cell model of SETBP1 disorder

Fig. 5

Genome-wide transcriptome profiling revealed SETBP1 regulation of Wnt signaling. A Principle component analysis (PCA) of the samples. B Heatmap depicting correlation with Allen Brain atlas at early, mid and late gestational trimesters and prenatal trimester. C Heatmap depicting 17,654 differentially expressed transcripts at day 34 (padj ≤ 0.1). D Example of neurogenic and proliferation related genes differentially expressed at day 34. E Example of neuronal marker genes downregulated at day 34, and upregulation of neuronal marker NES. F, G Differentially expressed genes associated with canonical non-canonical Wnt pathway at day 34. H Representative images of Western blot analysis for Wnt signaling proteins for WT and SETBP1-/- (Homo1). I Relative protein level of B-catenin, B-catenin p-S552 and p-S675, and LRP6 co-receptor and p-LRP6 at day 21, 30 and 40 to WT basal levels. Data from 3 independent differentiations analysed in duplicates or triplicates. Student’s T test was used to compare the expression between the two lines. (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). All tested proteins were normalized to GAPDH

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