Fig. 5

Synaptic changes in hippocampus of the offspring of Het dams. A Fractionation of the hippocampus. Western Blots of Sal-WT and Pol-KO are shown for SHANK3, PSD95 (postsynaptic) and SYNAPTOPHYSIN (SYN, presynaptic). B Western Blot analysis for SHANK3 and β-ACTIN of hippocampus homogenate. The SHANK3e isoform was analyzed. G:T ***p = 0.0007, G *p = 0.0166, T ****p < 0.0001, Sal-WT vs Pol-KO **p = 0.0017, Sal-Het vs Pol-KO ****p < 0.0001, Sal-KO vs Pol-KO ****p < 0.0001, Pol-WT vs Pol-KO #p = 0.0591, Pol-Het vs Pol-KO **p = 0.0038, Sal-WT vs Sal-KO #p = 0.0897. C Western Blot analysis for SHANK3 and β-ACTIN of hippocampus P3 fraction. The SHANK3e isoform was analyzed. G:T ****p < 0.0001, G #p = 0.0681, Sal-WT vs Sal-Het *p = 0.0429, Sal-WT vs Sal-KO **p = 0.0020, Sal-WT vs Pol-WT *p = 0.0484, Sal-WT vs Pol-Het #p = 0.0543, Pol-KO vs Sal-KO **p = 0.0013. D Western Blot analysis for SHANK2 and β-ACTIN of hippocampus homogenate. The total SHANK2 was analyzed. G:T **p = 0.0035, G #p = 0.0533, T #p = 0.0515, Sal-WT vs Sal-Het *p = 0.0317, Sal-WT vs Sal-KO #p = 0.0565, Sal-Het vs Pol-KO *p = 0.0172, Sal-KO vs Pol-KO *p = 0.0304. E Western Blot analysis for SHANK2 and β-ACTIN of hippocampus P3 fraction. The total SHANK2 was analyzed. G:T ***p = 0.0006, G *p = 0.0309, T *p = 0.0470, Sal-Het vs Pol-KO **p = 0.0042, Sal-KO vs Pol-KO **p = 0.0021, Pol-WT vs Pol-KO *p = 0.0123, Pol-Het vs Pol-KO **p = 0.0082. F Western Blot analysis for mGluR5 and β-ACTIN of hippocampus homogenate. G Western Blot analysis for mGluR5 and β-ACTIN of hippocampus P3 fraction. G:T ***p = 0.0004, Sal-WT vs Sal-Het *p = 0.0117, Sal-WT vs Sal-KO *p = 0.0124, Sal-WT vs Pol-WT *p = 0.0333, Sal-KO vs Pol-KO *p = 0.0396. H Western Blot analysis for HOMER1b/c and β-ACTIN of hippocampus homogenate. T *p = 0.0256, Pol-WT vs Sal-WT *p = 0.0439. I Western Blot analysis for HOMER1b/c and β-ACTIN of hippocampus P3 fraction. G:T ****p < 0.0001, G **p = 0.0010, Sal-WT vs Sal-Het ***p = 0.0007, Sal-WT vs Sal-KO ***p = 0.0004, Sal-WT vs Pol-WT **p = 0.0012, Sal-WT vs Pol-Het ***p = 0.0003, Sal-KO vs Pol-KO *p = 0.0236. J Western Blot analysis for PSD95 and β-ACTIN of hippocampus homogenate. T #p = 0.0581, Pol-WT vs Sal-WT #p = 0.0904. K Western Blot analysis for PSD95 and β-ACTIN of hippocampus P3 fraction. B–K Data were tested for normality with the Shapiro–Wilk test followed by two-way ANOVA with a Bonferroni correction for multiple comparisons. Significance level was set to 0.05 (# < 0.10, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Mean ± SEM, n = 3. G Genotype of the offspring; T Treatment of the mother; G:T interaction between the two factors; and ns not significant