Fig. 4

Synaptic changes in striatum of the offspring of Het dams. A Fractionation of the striatum. Western Blots of Sal-WT and Pol-KO are shown for SHANK3, PSD95 (postsynaptic) and SYNAPTOPHYSIN (SYN, presynaptic). B Western Blot analysis for SHANK3 and β-ACTIN of striatum homogenate. The SHANK3e isoform was analyzed. G:T **p = 0.0021; T *p = 0.0227, Pol-KO vs Sal-KO *p = 0.0114, Sal-WT vs Sal-KO *p = 0.0301, Sal-WT vs Sal-Het #p = 0.0756. C Western Blot analysis for SHANK3 and β-ACTIN of striatum P3 fraction. The SHANK3e isoform was analyzed. G:T **p = 0.0040, Pol-KO vs Sal-KO #p = 0.0842, Sal-KO vs Sal-WT *p = 0.0155. D Western Blot analysis for SHANK2 and β-ACTIN of striatum homogenate. The total SHANK2 was analyzed. G:T **p = 0.0075, G #p = 0.0585, T ***p = 0.0004, Pol-KO vs the following: Sal-WT **p = 0.0052, Sal-Het **p = 0.0018, Sal-KO **p = 0.0010, Pol-WT *p = 0.0173, Pol-Het *p = 0.0440. E Western Blot analysis for SHANK2 and β-ACTIN of striatum P3 fraction. The total SHANK2 was analyzed. G:T *p = 0.0296, Sal-KO vs Pol-KO #p = 0.0510, Sal-WT vs Sal-KO #p = 0.0909. F Western Blot analysis for mGluR5 and β-ACTIN of striatum homogenate. G:T *p = 0.0236, T **p = 0.0028, Sal-Het vs Pol-KO *p = 0.0285, Sal-KO vs Pol-KO **p = 0.0091. G Western Blot analysis for mGluR5 and β-ACTIN of striatum P3 fraction. G:T **p = 0.0017, T *p = 0.0450, Sal-KO vs Pol-KO **p = 0.0058, Sal-WT vs Sal-KO #p = 0.0565. H Western Blot analysis for HOMER1b/c and β-ACTIN of striatum homogenate. G ***p = 0.0006, T ***p = 0.0010, Sal-WT vs Sal-KO **p = 0.0055, Pol-WT vs Pol-KO *p = 0.0221, Sal-WT vs Sal-Het *p = 0.0184, Pol-WT vs Pol-Het #p = 0.0702, Pol-Het vs Sal-Het #p = 0.0542, Pol-KO vs Sal-KO #p = 0.0505. I Western Blot analysis for HOMER1b/c and β-ACTIN of striatum P3 fraction. G:T #p = 0.0928, G **p = 0.0085, Sal-WT vs Sal-KO **p = 0.0032, Sal-Het vs Sal-KO #p = 0.0954. J Western Blot analysis for PSD95 and β-ACTIN of striatum homogenate. G:T #p = 0.0761, G *p = 0.0447, Sal-WT vs Sal-KO **p = 0.0084. K Western Blot analysis for PSD95 and β-ACTIN of striatum P3 fraction. G ***p = 0.0003, Sal-WT vs Sal-KO **p = 0.0042, Pol-WT vs Pol-KO **p = 0.0035. B–K Data were tested for normality with the Shapiro–Wilk test followed by two-way ANOVA with a Bonferroni correction for multiple comparisons. Significance level was set to 0.05 (# < 0.10, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Mean ± SEM, n = 3. G Genotype of the offspring; T Treatment of the mother; G:T interaction between the two factors; and ns not significant