Fig. 3
WT but not Fmr1−/y CA1 pyramidal neurons exhibit experience-dependent changes in mean firing rate and burst probability. A The mean firing rate of CA1 pyramidal neurons decreased significantly between the first and second day of exploration in the novel environment in WT but not in Fmr1−/y rats (genotype x day interaction p = 0.006; post hoc comparisons Day 1 vs Day 2, WT: p < 0.0001; Fmr1−/y: p = 0.131). There were no significant differences in mean firing rate between genotypes on either Day 1 or Day 2 (post hoc comparisons p’s > 0.05). B Burst probability of pyramidal neurons decreased significantly between Day 1 and Day 2 in WT, but not in Fmr1−/y rats (genotype x day interaction p = 0.005; post hoc comparisons Day 1 vs Day 2: WT: p < 0.0001; Fmr1−/y p = 0.924). There were no significant differences between genotypes on either day (post hoc comparisons p’s > 0.05). Data represent cell means and SEMs. Statistical analyses used linear mixed effect (LME) modelling with genotype, day and session-in-day as fixed factors, cell and rat as random factors, followed by Tukey comparisons on emmeans for significant interactions. NWT-D1 = 222 cells, NWT-D2 = 207 cells, NKO-D1 = 211 cells, NKO-D2 = 205 cells. Pale yellow and pale purple backgrounds denote data from Day 1 and Day 2, respectively