Fig. 6

Eye closure response to air puff differs between male, but not female, WT and Shank2-KO mice. A Eye closure responses (average of 5 trials) of adult male WT (black; n = 12) and Shank2-KO (red; n = 10) mice (shading, SEM across mice) to strong (100 ms, 3 psi; left), mild (5 ms, 3 psi; middle), and very strong (100 ms, 30 psi; right) air puffs. Shaded rectangles indicate time periods before (green, 1.5 s before air puff onset), during (orange, 1 s after air puff onset), and after air puff delivery (purple, between 2.5 and 4 s after air puff onset). B The difference in eye closure response between Shank2-KO and WT mice to 16 different combinations of air puff duration (abscissa) and pressure (ordinate) before (left), during (middle), and after (right) air puff delivery. C Eye closure responses of adult of female WT (black; n = 11) and Shank2-KO (red; n = 10) mice. The results are presented as described for panels A–C. *p < 0.05, **p < 0.01, using two-way mixed ANOVA followed by post hoc Bonferroni test