Fig. 5

Social approach and social novelty preference behaviors are differentially sensitive to sex, Shank3 genotype, and sleep disruption treatments. A Examples of individual WT and Shank3^WT/ΔC heterozygotes (HET) from control, ELSD, and PASD treatment groups performing the 3-chamber social approach task. Heat map indicates time in location. Mice can freely move between 3-chambers and choose to spend time engaging with the non-social stimulus (N) or with a social stimulus (S). B, C Social approach behavior: time spent in proximity with the non-social (N) and social stimulus (S) for WT and HET males (B) or females (C). ELSD HET males show no preference for the social stimulus. D, E Social novelty preference: time spent in proximity with familiar stranger 1 (S1) and novel stranger 2 (S2) for WT and HET males (D) or females (E). PASD HET males and WT females show no preference for S2. HET females show now preference for S2 under any condition tested. *P < 0.05, **P < 0.01, **P < 0.001 (paired t test with Holm–Šídák correction). N = 8–12 per treatment/sex/genotype. Summary of statistical analysis is shown in Additional file 2