Fig. 3

ELSD drives sex-specific changes in certain non-social behaviors in Shank3^WT/ΔC heterozygotes. A–D Percent open arm time and entries for WT and Shank3^WT/ΔC heterozygotes (HET) from control, ELSD, and PASD treatment groups. A Male % time spent in the open arms. B Male % open arm entries. No differences were detected in any male groups. C Female % time spent in the open arms. ELSD HET females spend significantly more time in the open arms than WT littermates. D Female % open arms entries. **P < 0.01 (unpaired t test for genotype with Tukey’s multiple comparisons test). E–F Total distance traveled in the open-field task for WT and HET males E or females F. ELSD HET males show hypo-activity in the open field test. G–H Percent pre-pulse inhibition (PPI) of acoustic startle responses for WT and HET males G or females H. ELSD HET males show reductions in PPI response. **P < 0.01 (Open field, unpaired t test with Tukey’s multiple comparisons; PPI, 2-way ANOVA with Šídák’s multiple comparisons test). Error bars indicate ± SEM. N = 8–12 mice per group/sex/genotype. Summary of statistical analysis is shown in Additional file 2. See also Additional file 4 (anxiety-like measures in open-field test)