Fig. 2

Early life or post-adolescent sleep disruption. A Experimental design. Cohorts of mice undergo 7 days of sleep disruption either from P14–P21: early life sleep disruption (ELSD), or from P56–P63: post-adolescent sleep disruption (PASD). Control cohorts are left undisturbed. Control, ELSD, and PASD cohorts include male and female WT and Shank3^WT/ΔC heterozygous littermates. Upon reaching adulthood (P70), control, ELSD, and PASD cohorts undergo a panel of behavioral testing lasting 6–7 weeks. B Control and ELSD pups were weighed daily from P14–P21. No differences were seen between groups (2-way ANOVA). N = 37 control pups; 39 ELSD pups. C Separate cohorts of control and ELSD treatment were killed at P21, and serum corticosterone (cort.) was measured using ELISA. No differences were seen between groups (unpaired t test). N  = 17 control, 33 ELSD. Error bars indicate ± SEM. See also Additional file 3