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Fig. 1 | Molecular Autism

Fig. 1

From: Imbalance of flight–freeze responses and their cellular correlates in the Nlgn3−/y rat model of autism

Fig. 1

Confirmation of the Nlgn3−/y rat model. A Base sequence of deletion in exon 5. Red text denotes exon, highlighted grey text denotes deletion location. B Schematic of potential truncated, full and spliced variants of the NLGN3 protein. Grey regions indicated amino acid sequence shared with the full-length isoform, dotted lines indicate a 17 amino acid section of the full-length Nlgn3 that is missing in the spliced form. C Nlgn3 mRNA expression in Nlgn3−/y animals expressed as a percentage of WT (WT n = 6, KO n = 5). D Percentage of Nlgn3−/y RNA-seq reads at the cryptic splice site which splice. (WT n = 6, KO n = 5). E Schematic illustrating antibody binding sites to WT NLGN3 protein. Western blot of cortical WT and Nlgn3−/y tissue using anti-NLGN3 N-terminus and anti-NLGN3 C-terminus. No NLGN3 protein of any form was found in Nlgn3−/y rats (WT n = 2, KO n = 2). Red arrow denotes expected band location if short isoform was present. No presence of the predicted short isoform was detected. Data represented as mean ± SEM, clear dots represent individual animals

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