Fig. 4
From: WDFY3 mutation alters laminar position and morphology of cortical neurons
Dendritic spine density increases with progressive Wdfy3 loss. A–C Representative 3D images of tdT and GFP immunolabeled neurons that are either wild type (A), heterozygous (B), or homozygous (C) mutant. D–F High-magnification images of individual dendritic spines. G Quantified and statistically analyzed spine density (mean ± SD) in basal and apical dendrites after the first bifurcation. Both + /lacZ (p ≤ 0.05) and lacZ/lacZ (p ≤ 0.01) neurons show significantly higher bouton density compared with WT. Homozygous mutant neurons also display higher bouton density compared with heterozygous neurons (p ≤ 0.05). H Bar diagram displaying the relative distribution of dendritic spines types found in analyzed cortical neurons. No significant differences were noted between genotypes with respect to spine subtype distribution. Scale bar is 20 μm