Fig. 2

Laminar distribution of labeled neurons reveals disproportionate misplacement of lacZ/lacZ neurons in Wdfy3-MADM-5 cortex. Immunofluorescent analysis reveals laminar positioning of WT and lacZ/lacZ neurons in relation to layers VI (Tbr1, A, B), V (CTIP2, C, D), and II-III (BRN2, E, F). G–I Quantifications of double-labeled cells reveal that, compared to WT, a significantly smaller percentage of lacZ/lacZ neurons is correctly positioned in their respective layer. The proportion of correctly placed layer VI (G) and layers II-III (I) lacZ/lacZ neurons significantly increases between P8 and P30, likely due to loss of misplaced neurons during early postnatal development. TBR1⁺/tdT⁺ vs. TBR1⁺/GFP⁺ at P8 p ≤ 0.0001 and at P30 p ≤ 0.001. CTIP2⁺/tdT⁺ vs. CTIP2⁺/GFP⁺ at P8 p ≤ 0.0001 and at P30 p ≤ 0.0001. BRN2⁺/tdT⁺ vs. BRN2⁺/GFP⁺ at P8 p ≤ 0.0001 and at P30 p ≤ 0.0001. Scale bar is 250 μm