Fig. 1

Genotype distributions in Wdfy3-MADM-5 cortex show specific loss of Wdfy3^lacZ/lacZ cells at early postnatal stages. A, B TdT and GFP immunofluorescence of somatosensory cortex at P8 (A) and P30 (B) show sparse neuronal and astrocytic labeling of expected genotypes, tdT⁺ cells are WT, GFP⁺ cells are lacZ/lacZ, and tdT⁺/GFP⁺ cells are heterozygous. The bar diagram in C depicts the changes in genotype distributions between the two developmental stages showing a significant relative reduction in GFP⁺ (lacZ/lacZ) neurons (p < 0.0001) and significant relative increases in tdT (WT; p = 0.02) and tdT⁺/GFP⁺ (+ /lacZ) neurons (p = 0.0002). Scale bar is 250 μm