Fig. 3
From: Autism-associated protein POGZ controls ESCs and ESC neural induction by association with esBAF
POGZ associates with esBAF. A Left: Cartoon showing the IP followed Mass Spec experiment. Right: representative POGZ interacting proteins identified. IP-Mass Spec experiments were repeated three times. B Co-IP results for FLAG-POGZ and the indicated proteins using FLAG-POGZ Pogz−/− ESCs. C Co-IP results confirming the interaction of endogenous POGZ and BRG1 in WT ESCs. D Co-IP results confirming the interaction of endogenous POGZ and BAF155 in WT ESCs. E Double IF staining of BRG1 and POGZ in WT ESCs. F Diagram showing the various truncated forms of BRG1 and POGZ used for mapping experiments. G Co-IP results showing that POGZ interacts with the C-terminal fragment of BRG1in 293 T cells. Up: IP POGZ; WB FLAG; Bottom: IP HA; WB POGZ. H POGZ-Cter pulled down the C-terminal fragment of BRG1 in 293 T cells. *: non-specific band. I HA-BRG1-Cter pulled down FLAG-POGZ-Cter in 293 T cells. J-K Co-IP results of in vitro synthesized POGZ and FLAG-BRG1-Cter. POGZ and FLAG-BRG1-Cter proteins were synthesized using a rabbit reticulocyte lysate system and subjected to pull-down analyses. Panel J: IP FLAG WB: POGZ; and panel K: IP POGZ WB: FLAG. All experiments were repeated at least two times, and shown are representative images