Fig. 2
From: Autism-associated protein POGZ controls ESCs and ESC neural induction by association with esBAF
POGZ promotes ESC neural induction. A Morphology of day 1, 3, and 6 EBs from control and early passage Mut1 ESCs. B Time course qRT-PCR analysis of indicated genes. C Volcano plot showing the up- and down-regulated genes (fold change > 2) of day 6 EBs from control and Pogz−/− ESCs. D Heat map showing the expression of indicated neural genes. Three replicates are shown: KO for Pogz−/− ESCs, and Ctr for control ESCs. E GO analysis of DEGs of day 6 EBs from control and Pogz−/− ESCs. F Morphology of day 2, 4, 7 neurospheres from control-, Pogz−/−, and POGZ-restoring ESCs. G Morphology of day 16 neuronal cells from control-, Pogz−/−, and POGZ-restoring ESCs. White arrows pointing to the neuronal fibers. H Time course qRT-PCR analysis of indicated neural genes during control and Mut1 ESC directional differentiation toward neural progenitors. I Flow showing the percentage of PAX6-positive cells in control and Pogz−/− ESC-derived neurospheres. J IF results showing PAX6-positive cells in day 7 neural progenitors from control, Mut1, and Mut2 ESCs. Bar: 25 μm. K Quantitation of (J) by Image J. Flow and IF experiments were repeated at least two times. qRT-PCR was repeated at least three times