Fig. 8
From: Myt1l haploinsufficiency leads to obesity and multifaceted behavioral alterations in mice
Effects of Myt1l haploinsufficiency on anxiety-related behavior, social behavior, interaction-induced ultrasonic vocalizations, self-grooming behavior, spatial working memory, and nest building. A Distance traveled in the open field. ANOVA with the between-subject factors genotype (G) and sex (S). S: F1,52 = 8.198; p = .006; all other p > 0.050. B Time spent in the center of the open field. ANOVA with the between-subject factors genotype (G) and sex (S). S: F1,52 = 18.534; p < .001; all other p > 0.050. C Time spent in the open arms of the elevated plus maze. ANOVA with the between-subject factors genotype (G) and sex (S). G: F1,52 = 8.747; p = .005; S: F1,52 = 7.494; p = .008; all other p > 0.050. D Entries into open and closed arms of the elevated plus maze. ANOVA with the between-subject factors genotype (G) and sex (S). S: F1,52 = 5.382; p = .024; all other p > 0.050. E, F Emission of female-induced ultrasonic vocalizations is not altered in Myt1l+/- mice. Representative spectrograms showing sequences of female-induced ultrasonic vocalizations (A: Myt1l+/+ littermate control, B: Myt1l+/- mouse). Please note the presence of low-frequency noise due to locomotor activity. G Social approach behavior is intact in Myt1l+/- mice. Repeated-measures ANOVA with the between-subject factors genotype (G) and sex (S) and the within-subject factor preference (P). P: F1,52 = 45.199; p < .001; all other p > 0.050. H Call number is not altered during male–female social interaction in Myt1l+/- mice. ANOVA with the between-subject factor genotype (G); all p > 0.050. I Call number is not altered during female–female social interaction in Myt1l+/- mice. ANOVA with the between-subject factor genotype (G); all p > 0.050. J Self-grooming. ANOVA with the between-subject factors genotype (G) and sex (S); all p > 0.050. K Alternation quotient Y-maze. ANOVA with the between-subject factors genotype (G) and sex (S); all p > 0.050. L Nest width. Repeated-measures ANOVA with the between-subject factors genotype (G) and sex (S) and the within-subject factor time (T), followed by unpaired t tests when appropriate. T: F1,260 = 25.511; p < .001; G: F1,52 = 5.591; p = .022; all other p > 0.050. M Nest quality. ANOVA with the between-subject factors genotype (G) and sex (S). G: F1,52 = 9.644; p = .003; all other p > 0.050. All data are means ± SEM, combined across males and females if not otherwise indicated; *p < 0.05; **p < 0.01; ***p < 0.001. #p < 0.05 versus 50% chance level