Fig. 2

eEF2K deletion protects Scn1a ± mice from epileptic seizure onset. A Representative EEG traces (a representative 60 min. registration is shown) of a WT, Scn1a ± and Scn1a ± eEF2K−/− mice in basal condition (left) and 7 days after thermal stress (right). Scn1a ± mice show high number of EEG spikes in basal and 7 days after thermal stress condition when compared with WT and Scn1a ± eEF2K−/− mice. B Quantification of the number of EEG spikes per 24 h in basal condition (WT n = 4, Scn1a ± n = 6 and Scn1a ± eEF2K−/− n = 5) and 7 days after heat (Scn1a ± n = 5 and Scn1a ± eEF2K−/− n = 4). Scn1a ± eEF2K−/− mice are clearly less susceptible to seizure in basal and 7 days after thermal stress condition than Scn1a ± mice. Data are presented as mean ± SEM. Statistical analysis for number of EEG spikes in basal condition **p < 0.01 versus corresponding WT, $$p < 0.01 versus corresponding Scn1a ± ; One-way ANOVA test, Tukey’s post hoc. Statistical analysis for number of EEG spikes 7 days after thermal stress condition $$p < 0.01 versus corresponding Scn1a ± ; Unpaired two-tailed t-test. Statistical analysis £p < 0.05 versus corresponding Scn1a ± in basal condition; Unpaired two-tailed t-test. C Temperature reached at the first seizure. eEF2K deletion increase the minimum temperature necessary to reach the first seizure in Scn1a ± mice. Data are presented as mean ± SEM. Scn1a ± n = 7, Scn1a ± eEF2K−/− n = 5. Statistical analysis $p < 0.05 versus corresponding Scn1a ± ; Unpaired two-tailed t-test. D Time before the first seizure occur at 40 °C. eEF2K deletion increase the time before the first seizure occurred or until 42.5 °C was reached. Data are presented as mean ± SEM. Scn1a ± n = 7, Scn1a ± eEF2K−/− n = 5. Statistical analysis $$p < 0.01 versus corresponding Scn1a ± ; Unpaired two-tailed t-test