Fig. 5
Transcriptomic survey of PtenY68H/+ cortex identifies broad changes in expression, affecting PTEN signaling and neurological processes, including oxytocin overexpression. a Volcano plot highlighting genes showing highly significant changes in differential expression (DE) in red (P < 0.0001; NWT = 6; NY68H = 5). b Heatmap of DE genes (threshold: P < 0.001) clustered by column (i.e., by expression pattern within a gene). c Top “Canonical Pathways” identified by Ingenuity Pathway Analysis (IPA) from DE gene list input. d Top identified IPA network from input DE gene list. The network is organized hierarchically. Red = overexpression; green = underexpression; solid line = direct relationship; hashed line = indirect relationship; arrow direction = direction of relationship. e STRING analysis of subnetwork of DE genes displaying the relationship between overexpressed Oxt and other DE genes (i.e., interactome). Node size trends with degree connectivity. Node color trends with betweenness centrality (i.e., cooler colors indicated higher betweenness centrality). Thickness of edge trends with the confidence in the biological relationship. f An oxytocin regulatory network constructed using IPA’s Grow and Molecule Activity Predictor (MAP) tools. Blue = predicted inactivation; orange = predicted activation; solid line = direct relationship; hashed line = indirect relationship; arrow direction = direction of relationship