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Fig. 4 | Molecular Autism

Fig. 4

From: Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity

Fig. 4

In vitro validation of PtenY68H microglia pathology. a Representative immunofluorescence staining of Pten+/+ and PtenY68H/+ primary microglia with Pten (red), Iba1 (red), C1q (green), Trem2 (green), Cx3cr1 (green), and DAPI (blue). N = 7. b-e Quantification of IF staining in panel a for Pten (P = 0.0006; b), Iba1 (P = 0.038; c), C1q (P = 0.004; d), Trem2 (P = NS; e), and Cx3cr1 (P = NS; f). g Representative image of phagocytosis assay comparing Pten+/+ and PtenY68H/+ microglia. h Quantification of panel g for proportion of active phagocytic microglia over total microglia. P = 0.01 i Quantification of panel g of average bead counts per phagocytic microglia. P = 0.005. P value key: *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Autism

ISSN: 2040-2392