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Fig. 3 | Molecular Autism

Fig. 3

From: Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity

Fig. 3

Evidence for a microglial pathology in the PtenY68H/+ cortex. a Representative immunofluorescence staining of six-week-old Pten+/+ and PtenY68H/+ cortical microglia with Iba1 (green) and DAPI (blue). N = 3. b Quantification of individual microglia area from data represented in panel a (P = 0.007) c Iba1 expression assessed from IF staining from panel a experiments (P = 0.001). d Total Iba1-positive cells normalized to total DAPI-positive cells from panel a experiments (P = 0.42). e–h Relative expression assessed on cortical tissue from six-week-old Pten+/+ and PtenY68H/+ mice (N = 7) via qRT-PCR for C1q (P = 0.04; e), Cx3cr1 (P = 0.003; f), Trem2 (P = 0.02; g), and Dap12 (P = 0.03; h). i-j Western analysis on cortical tissue from six-week-old Pten+/+ and PtenY68H/+ mice (N = 8) for C1q (P = 0.01; i), Cx3cr1 (P = 0.001: j), and Bdnf (P = 0.004; k). P value key: *P < 0.05, **P < 0.01, ***P < 0.001

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