Fig. 2
Behavioral phenotyping of PtenY68H/+ mice. a Schematic of the experimental design for the three-chamber sociability and social novelty tests as they were performed on six-week-old Pten+/+ and PtenY68H/+ mice. b Quantification of the three-chamber sociability test, showing time spent in empty chamber or in chamber containing the social target (P < 0.0001; N+/+  = 13; NY68H/+  = 10). c Quantification of the three-chamber social novelty test performed with six-week-old Pten+/+ and PtenY68H/+ mice, showing time spent in chamber containing familiar or novel social target (P < 0.0001; N+/+  = 13; NY68H/+  = 10). d Representative image of marble burring test results for Pten+/+ and PtenY68H/+ mice. e Quantification of marble burying test, showing mean marbles buried between Pten+/+ and PtenY68H/+ mice (MedianΔMarbles = 4.68; 97% CI: 2.12–7.23; P = 0.002). f From open field test (N = 6), the amount of time (s) mice spent in center of field (P = 0.65). g Time in intermediate zone (s) assessed by open field test (P = 0.078). h Time in thigmotaxis (s) as assessed by open field test (P = 0.018). i Total distance traveled during open field testing (P = 0.16). j mean velocity of mice during open field testing (P = 0.26). k Rotarod testing experiment (N = 8; Pgenotype = 0.90; Plearning = 0.01). l Ultrasonic vocalization testing (N = 4; P = 0.057). Datapoints are colored by mouse sex. Males in blue. Females in red. P value key: *P < 0.05, **P < 0.01, ****P < 0.0001