Mind the translational gap: using iPS cell models to bridge from genetic discoveries to perturbed pathways and therapeutic targets

Table 2 Protocols for fast generation of specialized neurons from iPSC cells (selected studies)

Terminal cell type	Protocol method	References
Glutamatergic excitatory neurons and neural progenitors	NGN2 expression	Zhang et al. [154]
Glutamatergic excitatory neurons and neural progenitors	NGN2 expression and WNT/dual-SMAD inhibition	Nehme et al. [47]
GABAergic inhibitory neurons	Transient expression of TFs (Ascl1 and Dlx2)	Yang et al. [155]
Dopaminergic neurons	Transient expression of TFs (rLmx1a, rNurr1 or rPitx3)	Mahajani et al. [156]
Sensory peripheral neurons	Small molecule-mediated direct differentiation, followed by human epidermal keratinocytes-conditioned medium	Guimareãs et al. [157]
Interneurons	Small molecule-mediated direct differentiation	Maroof et al. [158]