Fig. 2

Hippocampal transcriptome sequencing analysis and functional annotation of differentially expressed genes. a Pie chart shows 787 up-regulated genes and 460 downregulated in Gabra4 knockout mice (n = 3 samples per genotype). b–d Autism spectrum disorder (b), epilepsy (c), and learning/memory (d) candidate genes are enriched in Gabra4^−/− differential expression genes. ***p < 0.0001, Fisher’s exact test; Error bars represent the standard error of the fraction, estimated using bootstrapping method with 100 resamplings. e, f The top enriched biological processes of GO terms (e) and KEGG pathways (f) with upregulated genes and down-regulated genes. The enrichment analysis was performed using DAVID bioinformatics tool with a p value cutoff of 0.05 and FDR less than 0.05