Fig. 4
Increased proliferation of basal neural progenitors in Chd8neo/neo embryos. a Immunohistochemistry to detect PH3B+  (blue) and TBR2+ (green) nuclei in coronal sections through the telencephalon of E14.5 embryos. White arrowheads indicate PH3B+/TBR2+nuclei. White box is shown as zoomed inset containing a double positive cell. b Quantification of TBR2+/PH3B+cells per 100 µm of neocortex in E14.5 embryos (+/+, n = 20; neo/+, n = 9; +/−, n = 7; neo/neo, n = 6; neo/−, n = 8; Mean ± SEM, *p < 0.05, ***p < 0.001, ANOVA followed by Tukey’s multiple comparisons test). c–e Cleaved caspase 3 (CC3) immunostaining of dorsal neocortex of E14.5 embryos. Black arrows indicate CC3+ cells and black box in E highlights area shown in zoomed inset containing a CC3+ cell. Scale bar = 100 μm. f Relative quantification of CC3+ cells in neocortex of E14.5 Chd8 mutant embryos, normalised to respective wildtype littermates (+/+, n = 17; neo/ + , n = 7; neo/neo, n = 6; neo/−, n = 11; Mean ± SEM **p < 0.01). Embryos used in these experiments were from 8 different litters