Fig. 1
From: Altered synaptic ultrastructure in the prefrontal cortex of Shank3-deficient rats
Disector method for estimation of synapse density. Electron micrographs showing examples of a reference layers with b their associated lookup layers. The orange masks indicate the postsynaptic spine of unique synapses, seen only in the reference layer or the lookup layer. The green masks show the postsynaptic spine of synapses seen in both layers. The red arrowheads indicate electron-dense PSDs. Note the split PSDs in the perforated synapses. Scale bar = 250 nm. WT, wild-type; Shank3-Het, Shank3 heterozygotes; Shank3-KO, Shank3 knockouts