Fig. 2
Identification of brain regions exhibiting distinct changes in neuronal activity upon social experience stimulation. a Representative images of c-Fos immunostaining in coronal brain sections from the indicated brain regions of control (Ctrl) or the indicated Shank mutant mice. Brain sections were obtained from mice housed in the naïve state or 1.5 h after tube tests or 1.5 h after social cooperation tests. Scale bar: 1 mm. b–g Quantification of activated neurons in the mPFC (ACC, PL and IL), striatum (DMS), nucleus accumbens (NAcc), habenula (MHb and LHb), lateral septum (LS), hippocampus (CA1, CA3 and DG) and periaqueductal gray (PAG) by c-Fos immunostaining in Shank2∆6–7 (b, d, f) and Shank3∆9 (c, e, g) mice. Both types of Shank mutant mice were compared with their respective WT littermates (Ctrl). (Ctrl/naïve, n = 11–24 slices (3 mice), Shank2∆6–7/naïve, n = 10–24 slices (3 mice); Ctrl/SD, n = 10–24 slices (4 mice), Shank2∆6–7/SD, n = 10–24 slices (4 mice); Control/SC, n = 11–24 slices (4 mice), Shank2∆6–7/SC, n = 9–24 slices (4 mice); Control/naïve, n = 9–13 slices (3 mice), Shank3∆9/naïve, n = 9–16 slices (4 mice); Control/SD, n = 9–24 slices (4 mice), Shank3∆9/SD, n = 11– 24 slices (4 mice); Control/SC, n = 12–24 slices (4 mice); and Shank3∆9/SC, n = 12–24 slices (4 mice); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; Mann–Whitney U test). Ctrl versus Shank2∆6–7/naïve/CA1, p = 0.0845; Ctrl versus Shank2∆6–7/naïve/CA3, p = 0.6047; Ctrl versus Shank2∆6–7/naïve/DG, p = 0.2230; Ctrl versus Shank2∆6–7/naïve/PAG, p = 0.9229; Ctrl versus Shank2∆6–7/naïve/ACC, p = 0.0222; Ctrl versus Shank2∆6–7/naïve/PL, p = 0.1987; Ctrl versus Shank2∆6–7/naïve/IL, p = 0.0008; Ctrl versus Shank2∆6–7/naïve/DMS, p = 0.0355; Ctrl vs Shank2∆6–7/naïve/NAcc, p = 0.0218; Ctrl vs Shank2∆6–7/naïve/LHb, p = 0.0020; Ctrl versus Shank2∆6–7/naïve/MHb, p = 0.0053; Ctrl versus Shank2∆6–7/naïve/LS, p = 0.0914; Ctrl versus Shank2∆6–7/SD/CA1, p = 0.2189; Ctrl versus Shank2∆6–7/SD/CA3, p = 0.6815; Ctrl versus Shank2∆6–7/SD/DG, p = 0.0403; Ctrl versus Shank2∆6–7/SD/PAG, p = 0.0295; Ctrl versus Shank2∆6–7/SD/ACC, p = 0.6505; Ctrl versus Shank2∆6–7/SD/PL, p = 0.7399; Ctrl versus Shank2∆6–7/SD/IL, p = 0.0129; Ctrl versus Shank2∆6–7/SD/DMS, p = 0.0005; Ctrl versus Shank2∆6–7/SD/NAcc, p = 0.4492; Ctrl versus Shank2∆6–7/SD/LHb, p = 0.0464; Ctrl versus Shank2∆6–7/SD/MHb, p = 0.4567; Ctrl versus Shank2∆6–7/SD/LS, p = 0.2657; Ctrl versus Shank2∆6–7/SC/CA1, p = 0.0278; Ctrl versus Shank2∆6–7/SC/CA3, p = 0.0992; Ctrl versus Shank2∆6–7/SC/DG, p = 0.0143; Ctrl versus Shank2∆6–7/SC/PAG, p = 0.0013; Ctrl versus Shank2∆6–7/SC/ACC, p = 0.0977; Ctrl versus Shank2∆6–7/SC/PL, p = 0.0134; Ctrl versus Shank2∆6–7/SC/IL, p = 0.0019; Ctrl versus Shank2∆6–7/SC/DMS, p = 0.0058; Ctrl versus Shank2∆9/SC/NAcc, p = 0.0003; Ctrl versus Shank2∆6–7/SC/LHb, p = 0.0098; Ctrl versus Shank2∆6–7/SC/MHb, p = 0.0482; Ctrl versus Shank2∆6–7/SC/LS, p = 0.4863; Ctrl versus Shank3∆9/naïve/CA1, p = 0.0093; Ctrl versus Shank3∆9/naïve/CA3, p = 0.0225; Ctrl versus Shank3∆9/naïve/DG, p = 0.5499; Ctrl versus Shank3∆9/naïve/PAG, p = 0.6771; Ctrl versus Shank3∆9/naïve/ACC, p = 0.0877; Ctrl versus Shank3∆9/naïve/PL, p = 0.0214; Ctrl versus Shank3∆9/naïve/IL, p = 0.0915 ; Ctrl versus Shank3∆9/naïve/DMS, p = 0.0225; Ctrl versus Shank3∆9/naïve/NAcc, p = 0.2523; Ctrl versus Shank3∆9/naïve/LHb, p = 0.7103; Ctrl versus Shank3∆9/naïve/MHb, p = 0.3693; Ctrl versus Shank3∆9/naïve/LS, p = 0.9279; Ctrl versus Shank3∆9/SD/CA1, p = 0.1978; Ctrl versus Shank3∆9/SD/CA3, p = 0.1600; Ctrl versus Shank3∆9/SD/DG, p = 0.2189; Ctrl versus Shank3∆9/SD/PAG, p = 0.0006; Ctrl versus Shank3∆9/SD/ACC, p = 0.0018; Ctrl versus Shank3∆9/SD/PL, p = 0.0001; Ctrl versus Shank3∆9/SD/IL, p = 0.0014; Ctrl versus Shank3∆9/SD/DMS, p < 0.0001; Ctrl versus Shank3∆9/SD/NAcc, p = 0.4746; Ctrl versus Shank3∆9/SD/LHb, p = 0.0099; Ctrl versus Shank3∆9/SD/MHb, p = 0.0036; Ctrl versus Shank3∆9/SD/LS, p = 0.0001; Ctrl versus Shank3∆9/SC/CA1, p = 0.8428; Ctrl versus Shank3∆9/SC/CA3, p = 0.1600; Ctrl versus Shank3∆9/SC/DG, p = 0.7125; Ctrl versus Shank3∆9/SC/PAG, p = 0.1600; Ctrl versus Shank3∆9/SC/ACC, p = 0.1432; Ctrl versus Shank3∆9/SC/PL, p = 0.1135; Ctrl versus Shank3∆9/SC/IL, p = 0.5137; Ctrl versus Shank3∆9/SC/DMS, p = 0.0004; Ctrl versus Shank3∆9/SC/NAcc, p = 0.0841; Ctrl versus Shank3∆9/SC/LHb, p = 0.5137; Ctrl versus Shank3∆9/SC/MHb, p = 0.7985; and Ctrl versus Shank3∆9/SC/LS, p = 0.6707. See also Additional file 1: Table S2 for summary