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Fig. 1 | Molecular Autism

Fig. 1

From: Pharmacological intervention to restore connectivity deficits of neuronal networks derived from ASD patient iPSC with a TSC2 mutation

Fig. 1

Changes in neuronal activity of patient and control neurons recorded with multi-electrode arrays (MEAs). a Representative voltage traces from three electrodes of the same MEA culture for control and TSC2 neurons at 40DPP (DPP = days post plating). Traces from TSC2 neurons show high levels of activity compared to control neurons, as expected from previous observations [17]. b Development of synchronised bursting across the array at 20, 30 and 40DPP for control (top) and TSC2 neurons (bottom). For each time point, upper panel shows a raster plot and lower panel shows an array-wide synchronised detection rate (ASDR) plot. Vertical-scale bars = 200 spikes per 200 ms bin, following 5 min of recording. c Basal excitability of control and TSC2 neurons showing average spike firing rate and number of single unit bursts detected. Changes in d synchronised burst (SB) activity and the number of spikes in individual SBs, e the spikes outside of SBs and f SB length and interval in control and TSC2 models. g Frequency distribution analysis represents the variation in the SB intervals of control neurons TSC2 neurons. h Connectivity correlation matrices heat map for the control and TSC2 neurons on the MEAs, and colours represent the correlation in the firing rates across the indicated electrode. Correlation matrices are calculated for 16 electrodes in control and TSC2 neurons plated on the MEAs. Values greater than zero represent positive correlation, while values below zero represent negative correlation. All plots show means ± SEM. *p < 0.05, **p < 0.01 following unpaired t-tests. Number of recorded wells = 6 for the control and 8 for TSC2 culture

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