Fig. 3

Laminar distribution of myelinated axons in the OFC of adults. Examples of Toluidine Blue staining viewed under the light microscope (a–l) and EM images (m–w) acquired from OFC sections. a–f, m–r Representative images from OFC layers 1–6 of adults with ASD. g–l, r–w Representative images from OFC layers 1–6 of neurotypical adults. Cross-sections of myelinated axons appear as ring or elongated oval shaped structures. The morphometrics and density of axon cross-sections were quantified and compared between ASD and Control groups. Arrows in e indicate examples of neuron somata. Arrowheads in k indicate examples of myelinated axon cross-sections