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Fig. 2 | Molecular Autism

Fig. 2

From: High-throughput screening identifies histone deacetylase inhibitors that modulate GTF2I expression in 7q11.23 microduplication autism spectrum disorder patient-derived cortical neurons

Fig. 2

NGN2-mediated conversion of iPSCs to iNs. a Timeline of differentiation protocol for iPSC-derived cortical neurons. ROCKi: ROCK inhibitor; Doxy: doxycycline; Puro: puromycin. b RT-qPCR analysis of Nestin and Synaptophysin mRNA expression (mean ± SE) in NGN2 neurons (WBS01CN3 line) at 2, 3, and 4 weeks of differentiation. The expression level is normalized against GAPDH, and further standardized to iPSCs levels. c Day 28 WBS01CN3 neurons express mature excitatory cortical neuron markers: NeuN, TUBB3, Synapsin 1/2, MAP2, VGLUT1 and SATB2. d mRNA levels of genes in the WBS region, BAZ1B, CLIP2, EIF4H and GTF2I (mean ± SD), in iPSC lines (left) and in NGN2-induced neurons (right) in the three genotypes (WBS, CTL, 7Dup) (n = 2). CTL: Ctl01C, Ctl08A; WBS: WBS01CN3, WBS02C; 7Dup: DUP01GN4, Dup02K. Relative expression was measured by RT-qPCR, to GAPDH and results were arbitrarily normalized to mRNA levels of CTL (asterisks indicate statistical significance according to a one-way ANOVA test: *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001)

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