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Table 1 Result of mass spectrometric analysis of Shank3 N-terminus postsynaptic interaction partners

From: Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus

Protein names

Gene names

Armadillo repeat protein deleted in velo-cardio-facial syndrome

Arvcf

14-3-3 protein sigma

Sfn

Catenin alpha-1

Ctnna1

Versican core protein

Vcan

Probable ubiquitin carboxyl-terminal hydrolase FAF-X

Usp9x

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3

Agap3

Pancreatic alpha-amylase

Amy2

SH3 and multiple ankyrin repeat domains protein 3

Shank3

Catenin delta-2

Ctnnd2

Dynactin subunit 1

Dctn1

Myosin phosphatase Rho-interacting protein

Mprip

Cadherin-2

Cdh2

F-box only protein 41

Fbxo41

Rho GTPase-activating protein 39

Arhgap39

Disks large-associated protein 4

Dlgap4

Synaptosomal-associated protein 47

Snap47

Claudin-11

Cldn11

Ras/Rap GTPase-activating protein SynGAP

Syngap1

Voltage-dependent N-type calcium channel subunit alpha-1B

Cacna1b

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

Agap2

Disks large homolog 3

Dlg3

SRC kinase signaling inhibitor 1

Srcin1

Catenin beta-1

Ctnnb1

Disks large homolog 4

Dlg4

Voltage-dependent R-type calcium channel subunit alpha-1E

Cacna1e

Catenin delta-1

Ctnnd1

Latrophilin-1

Lphn1

Sorbin and SH3 domain-containing protein 1

Sorbs1

Brain-specific angiogenesis inhibitor 2

Bai2

  1. The isolated postsynaptic fraction from mouse brain was subjected to affinity purification with either His6-SUMO/Shank3 N-terminal fusion proteins or His6-SUMO as negative control. Both sets of samples were analysed by mass spectrometry. The label free quantification (LFQ) intensity signals obtained from the negative control samples were divided by the LFQ intensity signals obtained from the Shank3 N-terminus purified samples. Partners with the highest residual LFQ intensity signals are listed. Proteins of the catenin and cadherin families are highlighted in yellow. A similar result was obtained in four separate purifications, two with the complete N-terminus (shown here) and two with the Ank repeats of Shank3 only
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Molecular Autism

ISSN: 2040-2392