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Table 1 Result of mass spectrometric analysis of Shank3 N-terminus postsynaptic interaction partners

From: Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus

Protein names Gene names
Armadillo repeat protein deleted in velo-cardio-facial syndrome Arvcf
14-3-3 protein sigma Sfn
Catenin alpha-1 Ctnna1
Versican core protein Vcan
Probable ubiquitin carboxyl-terminal hydrolase FAF-X Usp9x
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 Agap3
Pancreatic alpha-amylase Amy2
SH3 and multiple ankyrin repeat domains protein 3 Shank3
Catenin delta-2 Ctnnd2
Dynactin subunit 1 Dctn1
Myosin phosphatase Rho-interacting protein Mprip
Cadherin-2 Cdh2
F-box only protein 41 Fbxo41
Rho GTPase-activating protein 39 Arhgap39
Disks large-associated protein 4 Dlgap4
Synaptosomal-associated protein 47 Snap47
Claudin-11 Cldn11
Ras/Rap GTPase-activating protein SynGAP Syngap1
Voltage-dependent N-type calcium channel subunit alpha-1B Cacna1b
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 Agap2
Disks large homolog 3 Dlg3
SRC kinase signaling inhibitor 1 Srcin1
Catenin beta-1 Ctnnb1
Disks large homolog 4 Dlg4
Voltage-dependent R-type calcium channel subunit alpha-1E Cacna1e
Catenin delta-1 Ctnnd1
Latrophilin-1 Lphn1
Sorbin and SH3 domain-containing protein 1 Sorbs1
Brain-specific angiogenesis inhibitor 2 Bai2
  1. The isolated postsynaptic fraction from mouse brain was subjected to affinity purification with either His6-SUMO/Shank3 N-terminal fusion proteins or His6-SUMO as negative control. Both sets of samples were analysed by mass spectrometry. The label free quantification (LFQ) intensity signals obtained from the negative control samples were divided by the LFQ intensity signals obtained from the Shank3 N-terminus purified samples. Partners with the highest residual LFQ intensity signals are listed. Proteins of the catenin and cadherin families are highlighted in yellow. A similar result was obtained in four separate purifications, two with the complete N-terminus (shown here) and two with the Ank repeats of Shank3 only