Fig. 2
From: Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus
a Interaction of catenin proteins with Shank3. 293T cells coexpressing mRFP-Shank3 with GFP-/Emerald-tagged catenin variants were lysed and subjected to immunoprecipitation using RFP-trap. Input and precipitate samples were analysed by Western blotting. The result shows that β- and δ-catenin (but not α-catenin) bind to full length Shank3, while only δ-catenin binds to the Shank3 N-terminal fragment (SPN-Ank). b 293T cells expressing GFP-tagged versions of the proteins indicated were lysed (Input samples) and subjected to a pulldown assay using immobilized His6-SUMO fusion proteins of the complete Shank3 N-terminus (SPN-Ank), the SUMO control protein or the Ank repeats alone. δ-catenin shows strong, specific binding to the Shank3 N-terminus when compared to the other interaction partners; Fodrin binds to the isolated Ank domain only, whereas HCN1 does not show any binding neither to Ank nor to the SPN-Ank fragment