Fig. 1
From: Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus
Colocalization of catenin proteins with Shank3 in HEK cells. RFP-tagged Shank3 was expressed either alone (upper panel) or coexpressed with Emerald-tagged α-catenin (middle panel) or GFP-tagged δ-catenin (lower panel) in 293T cells, followed by fixation and confocal microscopic analysis. The results show Shank3 presenting in large nuclear and cytosolic clusters, as described previously [24]; no significant overlap between α-catenin and Shank3; and extensive colocalization of δ-catenin and Shank3 at the plasma membrane (while some δ-catenin/Shank3 clusters remain intracellular). Blue: DAPI staining shows nucleus (scale bar 10 µm)