Fig. 4

mGluR signaling induces rapid RNA and RPF changes in wild-type hippocampal slices. a, b Heat maps of mRNAs and RPFs that are responsive to DHPG (100 µM, 5 min) in wild-type hippocampal slices. Relative gene expression is shown; each column represents a biological replicate (n = 7 for RNA-Seq, n = 8 for ribosome profiling), differentially expressed RNA (n = 241, FDR < 0.01) and RPFs (n = 212, FDR < 0.05). c Examples of DHPG-responsive mRNAs (top) or RPFs (bottom) are shown with normalized RNA and RPF counts from DESeq2. p values are from the output of DESeq2 analysis (Wald-test with BH correction). d Empirical cumulative distribution function (ECDF) of RNA (left), RPF (middle), and TE (right) expression changes in DHPG/Basal (log2FC) of FMRP binding targets compared to nontarget mRNAs. FMRP binding targets are stratified into three groups based on binding stringency. Wilcoxon rank-sum test with correction by Bonferroni method. p values are indicated in the plot legend