Fig. 1

Successful maternal Ube3a gene activation upon Nestin-Cre-mediated deletion of the transcriptional STOP cassette. a, b Western blot data and UBE3A quantification from total lysates of E9 and E15 brains of Ube3a^mLSL+, Ube3a^mLSL/− mice, and WT littermates normalized to WT E15 levels. Bars depict mean ± SD (n = 2 per genotype, per time point). c, d DAB and immunofluorescent pictures of UBE3A expression in sections of brains from adult wild-type, Ube3a^mLSL/Cre⁺ and Ube3a^mLSL/Cre⁻ mice