Fig. 6

Rescue of synaptogenic function of NL4X variants. a Effect of 4PBA on the expression of NL4X variants that showed the impaired maturation. b Representative images of synaptogenic activity of NL4X variants treated with 4PBA. Scale bar 10 μm. c Quantitative result of synaptogenic activity of NL4X variants treated with 4PBA (n = 14–37, **p < 0.001 vs WT with vehicle by Tukey-Kramer multiple comparisons test; n.s., not significant). d Effect of INCB3619 on the surface expression of NL4X L593F variant expressed in HEK293 cells. e Quantitative analysis of the western blot for biotinylated WT and variants (n = 7, *p < 0.05, **p < 0.01 vs WT by one-way ANOVA with Tukey HSD post hoc analysis). f Representative images of synaptogenic activity of NL4X L593F variant treated with INCB3619. Scale bar 10 μm. g Quantitative result of synaptogenic activity of NL4X L593F variant treated with INCB3619 (n = 12–16, *p < 0.05, **p < 0.01 vs GFP by Tukey-Kramer multiple comparisons test)