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Fig. 1 | Molecular Autism

Fig. 1

From: Autism-associated variants of neuroligin 4X impair synaptogenic activity by various molecular mechanisms

Fig. 1

Metabolism of NL4X protein. a Schematic depiction of NL4X metabolism. Positions of ASD/XLMR-associated variants are indicated by arrow. Epitopes of antibodies are also shown. b Characterization of antibodies used in this study. c Protein metabolism of overexpressed NL4X in COS-1 cells. d Deglycosylation assay of overexpressed NL4X in COS-1 cells. e Cell surface biotinylation of overexpressed NL4X in COS-1 cells. f Detection of endogenous NL4X in IMR32 cells. g Immunoprecipitation of endogenous sNL4X secreted from IMR32 cells. h Deglycosylation assay of endogenous NL4X in IMR32 cells. i Detection of endogenous NL4X in iPSC-derived human neurons. j Immunoprecipitation of endogenous sNL4X secreted from iPSC-derived human neurons. k Immunoblot analysis of the lysates of the NL4X KO iPSC-derived human neurons

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