Fig. 3
From: Cerebral organoids as tools to identify the developmental roots of autism
Sections of (a) 30-day, (b) 45-day, and (c) 65-day-old cerebral organoid grown from wild-type human iPSCs. a Immunostaining for progenitor marker PAX6 (red) and deep layer neuronal marker CTIP2 (green). b Immunostaining for intermediate progenitor marker TBR2 (green), deep later neuronal marker TBR1 (red), and deep layer neuronal marker CTIP2 (white). c Immunostaining for progenitor marker PAX6 (yellow), deep layer neuronal marker CTIP2 (red), and upper layer neuronal marker SATB2 (green). Progenitors are located interiorly, near the central lumen whereas differentiated neurons are located towards the outer edge of the organoid and multiple time points show that neuronal layers are progressively established, resembling in vivo embryonic cortex. L, lumen, O, outer edge. Scale bar, 100 μm