Fig. 2

Transcriptional response to IGF-1 in iPSC-derived neurons from ASD individuals. a MA-plot of differential expression between neurons from ASD individuals incubated either in media with the addition of water or after exposure to acute (left) or chronic (right) IGF-1. logCPM log counts per million, logFC log fold-change. Red dots = genes p-adj < 0.05, grey dots = genes p-adj > 0.05. Numbers in the top and bottom corners indicate the number of transcripts differentially expressed with a logFC > 0 or logFC < 0. b Overlap in genes (transcripts) identified as differentially expressed with p-adj < 0.05 in the acute and chronic conditions. c Expression patterns of differentially expressed genes that were shared between acute and chronic conditions (left), only identified in the acute condition (middle), or only identified in the chronic condition (right). Y = TPM values scaled by gene. Number in corner = number of genes in each group. d Overlap between genes differentially expressed between ASD H2O and IGF-1 (acute or chronic) and genes that are differentially expressed between neurotypical controls (CTL) in H2O versus IGF-1 (acute or chronic). e Eigenvalue expression within WGCNA gene modules (grey, turquoise, and brown) that were significantly associated with the interaction of disease and IGF-1. n = gene count within each module