Fig. 1

Transcriptional response to IGF-1 in iPSC-derived neurons from neurotypical individuals. a Schematics of differentiation of ASD and control cells highlighting the chronic and acute timeline for recombinant human IGF-1 treatment and subsequent RNA isolation. b MA-plot of differential expression between neurons from neurotypical control individuals incubated either in media with the addition of water or after exposure to acute (left) or chronic (right) IGF-1. logCPM log counts per million, logFC log fold-change. Red dots = genes p-adj < 0.05, grey dots = genes p-adj > 0.05. Numbers in the top and bottom corners indicate the number of transcripts differentially expressed with a logFC > 0 or logFC < 0. c Overlap in genes (transcripts) identified as differentially expressed with p-adj < 0.05 in the acute and chronic conditions. d Expression patterns of differentially expressed genes that were shared between acute and chronic conditions (left), only identified in the acute condition (middle), or only identified in the chronic condition (right). Y = TPM values scaled by gene. Number in corner = number of genes in each group