Fig. 1
From: Transcriptional consequences of MBD5 disruption in mouse brain and CRISPR-derived neurons
Study design and comparison of mouse Mbd5 and human MBD5 gene and protein structure. a Left—representation of the mouse brain regions of the Mbd5+/GT mouse model14 analyzed (cerebellum, cortex, and striatum). Right—schematic representation of the mouse Mbd5 gene found on 2qC1.1, including the gene-trap cassette inserted within intron 214. Below, the canonical protein isoform 1, the main described isoform, composed of a conserved MBD, a proline-rich segment (P rich), a PWWP domain. b Left—human iPSC-derived NPCs and neurons were used to generate isogenic edited cell lines. Right—schematic representation of the human MBD5 gene structure found on 2q23.1, including the dual guideRNA strategy for CRISPR/Cas9 editing targeting exon 6. Below, protein isoform 1, the most expressed in brain