Fig. 3From: Cortical neurons derived from human pluripotent stem cells lacking FMRP display altered spontaneous firing patternsNeurons lacking FMRP display bouts of inward current activity of higher frequencies but of shorter durations compared to control neurons. a Representative voltage-clamp recording (Vhold = −70 mV) of spontaneous inward currents from either FMR1+/y (black) or FMR1−/y (red) neurons illustrating the difference in spontaneous events. The insets below, each trace illustrates an expanded time-base where evidence of synaptic currents underlying these inward currents can be observed. b As in a but illustration recordings from hiPSC lines CON1 (black) and FXS1 (red). c The number of events is higher in neurons lacking FMR1 than control. Overall, the mean number for bursts (per 10 min) are 4.5 ± 0.56 (FMR1+/y; n = 8, N = 3); 20.67 ± 3.96 (FMR1−/y; n = 12, N = 3); 2.8 ± 0.34 (CON1; n = 13, N = 3); 23.85 ± 2.92 (FXS1; n = 11, N = 3). d Mean burst durations recorded from neurons lacking FMRP are shorter than those from control lines. Overall, the mean durations are 40.72 ± 6.8 s (FMR1+/y); 9.28 ± 1.05 s (FMR1−/y); 59.90 ± 9.07 s (CON1); 11.52 ± 1.55 s (FXS1). ***p < 0.001, paired t test, Wilcoxon testBack to article page