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Fig. 3 | Molecular Autism

Fig. 3

From: Elevated de novo protein synthesis in FMRP-deficient human neurons and its correction by metformin treatment

Fig. 3

Metformin corrects the aberrantly elevated protein synthesis in FMRP-deficient neurons but does not normalize the phosphorylated levels of Akt and ERK. a Global protein synthesis was determined by SUnSET assay, and representative immunoblot image was shown. Metformin (0.5 mM) was added to the neurons for 72 h followed by puromycin incorporation for 30 min. Protein loading was determined by the level of Calnexin. Representative immunoblot image was shown, and relative puromycin expression was quantified by ImageJ. Values shown as mean ± SEM from three biological replicates per sample. Statistical significance was shown as *p < 0.05, **p < 0.01, as determined by one-way ANOVA. b Expressions of phosphorylated Akt, total Akt, phosphorylated ERK and total ERK for untreated and metformin-treated condition were assessed by immunoblot. Protein loading was determined by the level of Calnexin. Phosphorylated Akt/total Akt and phosphorylated ERK/total ERK were quantified by ImageJ. Values shown as mean ± SEM from three biological replicates per sample. Statistical significance was shown as *p < 0/05, **p < 0.01, as determined by one-way ANOVA

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