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Fig. 1 | Molecular Autism

Fig. 1

From: Tsc1 haploinsufficiency in Nkx2.1 cells upregulates hippocampal interneuron mTORC1 activity, impairs pyramidal cell synaptic inhibition, and alters contextual fear discrimination and spatial working memory in mice

Fig. 1

Heterozygous conditional knockout of Tsc1 in Nkx2.1 interneurons causes cell-specific increase in mTORC1 activity. a Representative western blots (left) and summary graph (right) showing unchanged S6 phosphorylation (p-S6 relative to total S6) levels in hippocampal lysates of Tg mice relative to control mice (n = 3 Wt (Nkx2.1wt/wt;Tsc1f/wt) and 5 Tg (Nkx2.1Cre/wt;Tsc1f/wt) mice, analyzed in duplicate; p > 0.05, Student’s t test). b, c Representative images (left) and summary graphs (right) showing increased p-S6 immunofluorescence in CA1 SOM (b) and PV (c) interneurons of Tg mice compared to control mice (n = 4 Wt and 6 Tg mice, two independent experiments; *p < 0.05, **p < 0.01, Student’s t test). Scale bar in c also applies to b. df Representative images (d) and summary graphs showing unchanged cell numbers (e) and soma area (f) of CA1 SOM (left) and PV (right) immunopositive interneurons of Tg mice compared to control mice (n = 4 Wt and 6 Tg mice for cell number; n = 3 Wt and 3 Tg mice for soma area; p > 0.05, Student’s t tests). Scale bar in d applies for all images

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