Fig. 6
From: Decreased nuclear Pten in neural stem cells contributes to deficits in neuronal maturation
Changes in Creb activation in Ptenm3m4 NSCs. a Western blot analysis of differentiated NSCs at 0, 3, 5, 7, and 10 DIV (depicted as rising black bar) without N2 growth factors. b Western blot analysis of differentiated NSCs at 0, 3, 5, 7, and 10 DIV (depicted as rising black bar) with N2 supplement (n = 3). c Transcript expression of Creb-regulated genes (Bdnf, Caln1, Nerl1b, and Rest) quantified by qRT-PCR at three timepoints: NSC, 7 DIV, and 10 DIV. For the NSC timepoint, Kruskal-Wallis testing found a significant difference in expression in Bdnf (p value = 0.025), Caln1 (p value = 0.0036), and Neur1b (p value = 0.011) among genotype groups, where Dunn’s multiple comparison testing identified a significant difference between mutant and wildtype expression of Caln1 (p value = 0.022) and Neur1b (p value = 0.034). For the 7 DIV timepoint, Kruskal-Wallis test found a significant difference in expression in Caln1 (p value = 0.011) and Neur1b (p value = 0.025) among genotype groups, where Dunn’s multiple comparison testing identified a significant difference between mutant and wildtype expression of Caln1 (p value = 0.034). For the 10 DIV timepoint, Kruskal-Wallis test found a significant difference in expression in Bdnf (p value = 0.025) and Caln1 (p value = 0.025) among genotype groups. Three biological replicates (n = 3) were performed for each genotype group at each timepoint and relative transcript expression was measured in 2−(∆∆Ct) (*p value < 0.05)