Fig. 5
From: Decreased nuclear Pten in neural stem cells contributes to deficits in neuronal maturation
Ptenm3m4 NSCs exhibit changes in neuronal activity and glia differentiation. a Western blot analysis (n = 3) assessing c-Fos, Tuj1, and NeuN expression in differentiated NSCs without specific lineage-driving growth factors (top panel) with quantification (bottom panel). Two-way ANOVA with Tukey-Kramer post hoc testing found significant decrease in c-Fos and NeuN in Ptenm3m4/m3m4 versus wildtype at 7 DIV (p value = 0.025; 0.021) and 10 DIV (p value = 0.034; 0.014). A significant increase in Tuj1 between Ptenm3m4/m3m4 and wildtype at 10 DIV was also observed (p value = 0.043). Two-way ANOVA with Tukey-Kramer post hoc testing found significant decrease in NeuN in Ptenm3m4/m3m4 versus wildtype at 10 DIV (p value = 0.0066). Several significant increase in Tuj1 between Ptenm3m4/m3m4 and wildtype at 3, 5, 7, and 10 DIV were also observed (p value =0.017; 0.0099; 0.025; 0.0002, respectively). b Western blot analysis (n = 3) shows c-Fos, Tuj1, and NeuN expression in differentiated NSCs treated with N2 supplement (top panel) with quantification (bottom panel). Densitometric quantification for specific protein expression is normalized to ß-actin. c Immunofluorescent staining at 10 DIV without growth factors for oligodendrocyte precursors cell (OPC), oligodendrocyte (OL), and myelination markers: Ng2 (red), Olig1 (green), Mbp (red), and Gfap (red), respectively (*p value < 0.05; **p value < 0.01; ***p value < 0.001; ****p value < 0.0001)