Fig. 3
From: Decreased nuclear Pten in neural stem cells contributes to deficits in neuronal maturation
Ptenm3m4 NSCs differentiation halts after becoming immature neurons. a Representative immunofluorescence staining for neuronal markers Tuj1 (green, n = 5), Dcx (green, n = 3), Map2 (green, n = 3), and NeuN (red, n = 3), showing increased presence of immature neurons in Ptenm3m4/m3m4 NSCs compared to wildtype NSCs when cultured in the absence of specific lineage-driving growth factors for 10 days. NeuN (mature neuron marker) expression is significantly lower in Ptenm3m4/m3m4 NSCs compared to their wildtype counterparts. b Quantification of immunofluorescence staining of cells expressing neuronal markers (Tuj1, Dcx, Map2, and NeuN) normalized to total number of nuclei (DAPI) per frame. Kruskal-Wallis testing, accompanied by Dunn’s multiple comparison testing, finding a significant increase in Tuj1 (p value = 0.0051) and Dcx (p value = 0.033) and a significant decrease in NeuN (p value = 0.015) comparing Ptenm3m4/m3m4 and wildtype NSCs (*p value < 0.05; **p value < 0.01)