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Fig. 1 | Molecular Autism

Fig. 1

From: Decreased nuclear Pten in neural stem cells contributes to deficits in neuronal maturation

Fig. 1

Characterized neural stem cells (NSCs) derived from Ptenm3m4/m3m4 mice have shown slow differentiation. a Immunofluorescence staining for stemness makers Pax6 (top panel), Sox2 (middle panel), and c-Myc (bottom panel) at 1 day in culture with growth factors. b Western blots for stemness markers Pax6, Sox2, and c-Myc over 5 days of random differentiation in the absence of growth factors. Representative blot (n = 3). c Cell counting assay to assess proliferation (n = 5). Ptenm3m4/m3m4 NSCs show increased proliferation at 5 DIV compared to wildtype as assessed by Kruskal-Wallis testing with Dunn’s multiple testing comparison (p value = 0.0012). d Immunofluorescence for Ki67 expression at 0, 3, and 5 DIV for all NSC genotypes. e Western blot analysis of undifferentiated NSCs (n = 3) for cell cycle progression markers (*p value < 0.05; **p value < 0.01; ****p value < 0.0001)

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