Fig. 5

Behavioral analyses after selective hippocampal downregulation of Mir146.a Fluorescent microscopy image of a coronal brain section collected 3 weeks after local stereotactic injections with AAV expressing shRNAmir for Mir146a gene. The construct expresses eGFP, which allow us to verify the site and the efficacy of infection. A Panoramic photo of an entire brain region was obtained by automatically aligning and stitching 50 tiled images into a mosaic (using the Zen Light Zeiss LSM software package). b–d Behavioral analyses (CFC (E), NOR (F), OLM (G)) performed in 3-month-old mice, 3 weeks after hippocampal stereotactic injections with either: AAV-Mir146a-shRNA or AAV-Scramble-shRNA. e Microtubule-associated protein doublecortin (DCX) immunohistochemistry showing a significantly lower number of DCX⁺ cells (mean number of DCX⁺ cells in the dentate gyrus (DG)/brain slides) in the DG of the mice locally injected with AAV-shRNA miR 146a in comparison to the one injected with AAV-shRNA scramble. All behavioral tests were performed on at least two independent experiments for each experiment: n6 mice per group. Results are given as mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, NS: not significant; by Student’s t test compared to 3-month-old mice littermates. All behavioral tests were performed on at least two independent experiments for each experiment: n6 mice per group. Results are given as mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, NS: not significant; by Student’s t test compared to 3-month-old mice littermates