Fig. 2

Altered neurogenesis in the Mir146a^−/− brains. a Sagittal section of E14.5 neocortex of Mir146a^+/+ and Mir146a^−/− brain stained with DAPI and the post-mitotic neuronal marker NeuN; white arrows show the differentiated neuron layer where the measurements were made. b Graph comparing the average thickness (±S.D.) of the NeuN⁺ cells layer between WT and mutant embryos; at least 3 images from comparable plane per mouse were used for the analysis; **p < 0.01 by Student’s unpaired 2-tailed t test. c Automated phase contrast image segmentation of neurites (pink) and cell body clusters (CBC in blue) of Mir146a^−/− neurons at day 3 using the Neurotrack software module. d Graph shows the total length of neurites (±S.E.M) of neurons from Mir146a^+/+ and (n = 7) Mir146a^−/− embryos (n = 6). Images were taken by the Incucyte S3 at 9 different spots per well (replicate) every 3 h during 6 days of culture; post analysis was performed automatically using the pre-designed mask; there are at least 4 technical replicates per embryo. e Graph shows the average of slopes (±S.D.) of neurite extension of corresponding graph d. **p < 0.01 by Wilcoxon ranked sum test