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Fig. 1 | Molecular Autism

Fig. 1

From: Absence of parvalbumin increases mitochondria volume and branching of dendrites in inhibitory Pvalb neurons in vivo: a point of convergence of autism spectrum disorder (ASD) risk gene phenotypes

Fig. 1

PV expression in 3–5 month old PV-EGFP mice. a Sagittal section showing PV+ Pvalb neurons (red) in various brain regions of a PV-EGFP mouse. Sections are co-stained with DAPI (blue) to identify cell nuclei. The analyzed regions 1–8 obtained by microscopy are shown at higher magnification and were obtained by confocal microscopy in (b); scale bar in (a) 1000 μm. b Confocal images of Pvalb neurons of a PV-EGFP mouse in the regions 1–8 stained for PV (upper row) and for EGFP (lower row), scale bar 50 μm. c Strong correlation between green EGFP signals (in a.u.) and red PV signals (in a.u.) in the 8 investigated regions indicates that EGFP signals are a reliable proxy measure of PV concentrations. Note the larger SD values for the PV staining than for EGFP staining in low-PV expressing Pvalb neurons (DG, CA3, CA1). d Representative standard curve using data from (c) of (corrected) EGFP signals vs. PV concentrations previously reported in (from low to high) hippocampal Pvalb neurons, PC, and cerebellar basket cells. e Estimation of PV concentrations in other Pvalb neuron subpopulations (for details, see suppl. material)

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Molecular Autism

ISSN: 2040-2392